The R1056 Zymo Quick-RNA Midiprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of up to 1 mg total RNA from cultured cells or tissue samples. The procedure combines a unique, single-step RNA extraction/binding buffer with Clean-Spin™ column technology to yield high-quality RNA in about 10 minutes. The Quick-RNA™ Midiprep Kit allows for the efficient recovery of total RNA from 103 to 108 cells or tissue. The method is easy: simply add the provided ZR RNA Buffer to extract total RNA from the cells of interest then purify the RNA using the provided Zymo-Spin™ V-E Columns. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
Order R1056 Zymo Quick-RNA™ MidiPrep Kit in the USA and Canada from MBP Inc.
|Equipment||Microcentrifuge, vortex, vacuum manifold|
|Purity||RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.|
|Sample Source||Cells or tissue samples, yeast, plant or bacteria.|
|Size Range||Total RNA ≥ 17 nt|
|Yield||Midiprep: 1 mg RNA (binding capacity), ≥ 200 µl (elution volume)|
Q1: What is the difference between the Quick-RNA Miniprep and the Quick-RNA Miniprep Plus?
Use the Quick-RNA Miniprep for cells and soft tissues. The “Plus” kit accommodates all sample types (cells, tissue, blood) and comes with DNA/RNA Shield (sample collection, transport, storage at ambient temperature).
Q2: Is DNase I available for individual purchase?
Yes, the catalog number for the DNase I set (DNase and DNA Digestion Buffer) that we offer is E1010.
Q3: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q4: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q5: I ran out of RNA Wash Buffer. Can I use something else?
Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
Q6: Will the kit isolate small RNAs?
Yes, this kit will isolate small/micro RNA’s ≥ 17 nucleotides.
Q7: Can samples be stored in RNA Lysis Buffer prior to processing?
Yes, samples in RNA Lysis Buffer are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q8: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Q9: Is it possible to extract proteins with the Quick-RNA kit?
Yes, proteins can be acetone precipitated from the column flowthrough. Please request supplementary protocol from Zymo Research Technical Support.