R1050 / R1051
Highlights
Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
DNA-Free: DNase I included.
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Description
The R1050 Zymo Quick-RNA Microprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 106) and tissue samples (up to 5 mg). The procedure combines a unique buffer system with Zymo-Spin? column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes. The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin? Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.
Equipment
Microcentrifuge, vortex
Purity
RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source
Cells or tissue samples, yeast, plant or bacteria. Compatible with DNA/RNA Shield and RNAlater.
Size Range
Total RNA ? 17 nt
Yield
Microprep: 10 ?g RNA (binding capacity), >6 ?l (elution volume)
Q1: What sample types is DNA/RNA Shield suitable for?
DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).
Q2: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q3: Is it possible to extract proteins with the Quick-RNA kit?
Yes, proteins can be acetone precipitated from the column flowthrough. Please request supplementary protocol from Zymo Research Technical Support.
Q4: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q5: Will the kit isolate small RNAs?
Yes, this kit will isolate small/micro RNA?s ? 17 nucleotides.
Q6: Can samples be stored in RNA Lysis Buffer prior to processing?
Yes, samples in RNA Lysis Buffer are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q7: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Q8: What is the difference between the Quick-RNA Miniprep and the Quick-RNA Miniprep Plus?
Use the Quick-RNA Miniprep for cells and soft tissues. The ?Plus? kit accommodates all sample types (cells, tissue, blood) and comes with DNA/RNA Shield (sample collection, transport, storage at ambient temperature).
Q9: I ran out of RNA Wash Buffer. Can I use something else?
Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
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99 / 100 Bioz Stars |
Targeting p53-dependent stem cell loss for intestinal chemoprotection
Sci Transl Med Published 07 Feb 2019
‘Fresh mucosal scrapings from 10 cm of jejunum were washed in cold PBS, resuspended in 700 ?l of RNA lysis buffer, and homogenized in a Dounce homogenizer. . RNA was isolated using the Quick-RNA MiniPrep kit (Zymo Research) according to the manufacturer?s instructions.. Organoids were freed from Matrigel using Cell Recovery Solution (Corning), as described in , before RNA isolation.’
Cellular acidosis triggers human MondoA transcriptional activity by driv…
‘A list of plasmids used, the vector backbone and their source is provided in the Key Resources Table.. Total cellular RNA was extracted using a Quick RNA Miniprep Kit (Zymo Research) according to manufacturer?s recommendations. cDNA was synthesized from 200 ng mRNA using the GoScript Reverse Transcription System (Promega) with oligo-dT primers.. A 100-fold dilution was used in a PCR reaction containing SYBR Green and analyzed on a CFX Connect Real Time System.’
Promotion of Myoblast Differentiation by Fkbp5 via Cdk4 Isomerization
Cell Rep Published 29 Jan 2019
‘Secondary antibodies were diluted in 5% milk in TBST and six subsequent washes were done with TBST.. RNA was extracted from cells using a Quick RNA Microprep (Zymo Research, R1051) or RNeasy Plus Mini (QIAGEN, 74136) kit, depending on cell number.. RNA quantity and purity were assessed using a NanoDrop Lite spectrophotometer (Thermo Fisher Scientific). cDNA was synthesized with ProtoScript II ‘
“RNA isolation from tissue culture cells used to be my most hated protocol – labor intensive, tedious, and the horrible smell of the BME used in the protocol. This kit is much faster and easier and no horrible smells! Both the RNA yield and the real time results are just as good as our previous kit.”
– Lisa G. (University of Virginia)
?Amazing results, the only RNA extraction kit I ever buy now. In my opinion, there is no product on the market that is as good a value and as effective as this kit for plant tissue RNA extractions.?
– Erin B. (Occidental College)
“Good price and easy to use, plus good quality. It is compatible to QIAGEN products RNeasy kit, but with reasonable price and equal quality. Especially the DNase is inexpensive and is included in the kit. No matter animal RNA, or plant RNA, or bacterial RNA, all could use with this one kit.”
– Xiaolu J. (Florida Atlantic University)
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