The R213 Zymo Quick-RNA MagBead kit at MBP Inc. provides a high-throughput, magnetic bead-based purification of high-quality total RNA (including small/microRNAs) from any sample source (e.g., cells, solid tissue, whole blood, biological fluids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples)), samples stored in DNA/RNA Shield, etc. The provided DNA/RNA Shield inactivates infectious agents and is ideal for sample storage at ambient temperatures. Total RNA is eluted into ≥50 µl of DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing, RT/PCR, hybridization, etc.
|Binding Capacity||15 µg total RNA per 30 µl MagBinding Beads.|
|Equipment Needed||Magnetic stand or separator, heat block, liquid handler or robotic sample processor (user provided).|
|Purity||High-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc.|
|Recommended Materials||(available separately) – 96-well Collection Plate (C2002; capacity is up to 1.2 ml/well), 96-Well Block (P1001; capacity is up to 2 ml/well), 96-well Elution Plate (C2003), Cover Foil (C2007), ZR-96 MagStand (P1005).|
|Sample Preservation||DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures (page 7).|
|Sample Sources||Any cells, solid tissue, whole blood, biological fluids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), samples stored in DNA/RNA Shield, etc.|
|Size Limits||Total RNA including small/microRNAs ≥ 17nt.|
|Storage||RNA eluted with DNase/RNase-Free Water (provided) can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage.|
Q1: What is the average RNA yield? (chart)
|Input||Average RNA Yield|
|Cells||10 µg (per 106 cells)|
|High Yield Tissue (mouse)||≥ 30 µg (per 10 mg)|
|Low Yield Tissue (mouse)||≤ 30 µg (per 10 mg)|
|Brain, Heart||5-15 µg|
|Whole Blood||(per 1 ml)|
Q2: How to improve purity, RIN scores and eliminate contamination (i.e., A260/230, A260/280 ratios, DNA, phenol, protein, salt, etc.)?
Purity, RIN and/or any type of contamination can result from initial sample preparation (i.e., inefficient lysis of the sample). To improve, increase the volume of the lysis reagent (e.g., TRI Reagent/TRIzol or RNA Lysis Buffer).
Q3: How to improve RNA yield?
- To ensure complete lysis, increase the volume of the lysis reagent (i.e., increase or titrate the volume of TRI Reagent/TRIzol or RNA Lysis Buffer). Lysate should be clear (not opaque or viscous). Pellet debris by centrifugation (if needed) and process the cleared supernatant.
- Prior to adding TRI Reagent/TRIzol or RNA Lysis Buffer, perform enzymatic treatment (Proteinase K; #D3001-2-20) and/or mechanical homogenization (bead beating with ZR BashingBead Lysis Tubes; #S6003) in DNA/RNA Shield (#R1100).