The R2014 Zymo Quick-RNA Fungal/Bacterial Microprep Kit is designed for the simple and rapid isolation of RNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state-of-the-art, ultra-high-density BashingBeads. Zymo-Spin column technology is then used to isolate the RNA that is ideal for downstream molecular-based applications including Next-Gen sequencing, RT-PCR, microarray, hybridization, etc.
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| Applicable For | For all sensitive downstream applications, such as Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc. |
|---|---|
| Elution Volume | 25 µl |
| Equipment | Microcentrifuge, vortex, cell disrupter/pulverizer |
| Processing Time | ≤ 10 minutes |
| Processing Volume | 50-100 mg (wet weight) fungi or bacteria. 109Â bacterial cells and 108Â yeast cells |
| Purity | RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8. |
| Sample Source | Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled. |
| Size Range | Total RNA ≥ 17 nt |
| Type | Total RNA |
| Yield | Miniprep 50 µg RNA (binding capacity) ≥ 25 µl (elution volume) |
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