The R105 Zymo Quick-RNA 96 Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cells (up to 106) and tissue samples (up to 5 mg). The procedure combines a unique buffer system with Zymo-Spin™ plate technology to yield high-quality total RNA (including small RNAs ~17-200 nt) in about 30 minutes. The procedure is simple: Add the provided RNA Lysis Buffer to a sample, then purify the RNA using the provided Silicon-A Plate. The result is highly concentrated, DNA-free RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, sequencing etc.
| Equipment | Centrifuge/rotor compatible with 96-well plates |
|---|---|
| Purity | RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. |
| Sample Source | Cells or tissue samples, yeast, plant or bacteria. Compatible with DNA/RNA Shield and RNAlater. |
| Size Range | Total RNA ≥ 17 nt |
| Yield | 10 µg RNA (binding capacity) ≥ 25 µl (elution volume) |
| Supplemental Info |
Q1: What is the difference between the Quick-RNA Miniprep and the Quick-RNA Miniprep Plus?
Use the Quick-RNA Miniprep for cells and soft tissues. The “Plus” kit accommodates all sample types (cells, tissue, blood) and comes with DNA/RNA Shield (sample collection, transport, storage at ambient temperature).
Q2: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q3: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q4: Is DNase I available for individual purchase?
Yes, the catalog number for the DNase I set (DNase and DNA Digestion Buffer) that we offer is E1010.
Q5: I ran out of RNA Wash Buffer. Can I use something else?
Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
Q6: Will the kit isolate small RNAs?
Yes, this kit will isolate small/micro RNA’s ≥ 17 nucleotides.
Q7: Can samples be stored in RNA Lysis Buffer prior to processing?
Yes, samples in RNA Lysis Buffer are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q8: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Q9: Is it possible to extract proteins with the Quick-RNA kit?
Yes, proteins can be acetone precipitated from the column flowthrough. Please see the Protein Purification appendix in the protocol.
Q10: What is the average RNA yield? (chart)
| Input | Average RNA Yield |
|---|---|
| Cells | 10 µg (per 106 cells) |
| HeLa | 15 µg |
| High Yield Tissue (mouse) | ≥ 30 µg (per 10 mg) |
| Spleen | 30-50 µg |
| Liver | 40-60 µg |
| Low Yield Tissue (mouse) | ≤ 30 µg (per 10 mg) |
| Brain, Heart | 5-15 µg |
| Muscle | 5-20 µg |
| Lung | 10-20 µg |
| Intestine | 10-30 µg |
| Kidney | 20-30 µg |
| Whole Blood | (per 1 ml) |
| Porcine | 10-20 µg |
| Human | 2-10 µg |
Q11: How to improve purity, RIN scores and eliminate contamination (i.e., A260/230, A260/280 ratios, DNA, phenol, protein, salt, etc.)?
Purity, RIN and/or any type of contamination can result from initial sample preparation (i.e., inefficient lysis of the sample). To improve, increase the volume of the lysis reagent (e.g., TRI Reagent/TRIzol or RNA Lysis Buffer).
Q12: How to improve RNA yield?
- To ensure complete lysis, increase the volume of the lysis reagent (i.e., increase or titrate the volume of TRI Reagent/TRIzol or RNA Lysis Buffer). Lysate should be clear (not opaque or viscous). Pellet debris by centrifugation (if needed) and process the cleared supernatant.
- Prior to adding TRI Reagent/TRIzol or RNA Lysis Buffer, perform enzymatic treatment (Proteinase K; #D3001-2-20) and/or mechanical homogenization (bead beating with ZR BashingBead Lysis Tubes; #S6003) in DNA/RNA Shield (#R1100).
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