The D3061 Zymo Quick-DNA Urine Kit is an innovative product designed for easy, reliable, and rapid isolation of cellular and/or cell-free DNA from up to 40 ml of urine. The product features a uniquely formulated urine DNA stabilization reagent that also functions as a precipitation reagent. After collection, total (cellular and cell-free) or cell-free urine can be stored at ambient temperature for up to one month by adding the Urine Conditioning Buffer. When ready to extract the urine DNA, just add the Clearing Beads, vortex, and centrifuge to collect the precipitate. Following precipitation, chemical lysis and enzymatic digestion are used to extract DNA from the precipitate. The DNA is subsequently purified and concentrated using Zymo-Spin IC-S Columns. Urine DNA isolated with the Quick-DNA Urine Kit is ideal for qPCR, array, methylation analysis, and other downstream applications.
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| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation sequencing. |
|---|---|
| Equipment | Centrifuge, microcentrifuge, and heat block/bath. |
| Processing Volume | ≤ 40 ml |
| Purity | Typical A260/A280 ≥ 1.8 |
| Sample Source | Urine |
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. |
| Size Range | 100 bp – 23 kb |
| Type | Total DNA including cfDNA |
| Yield | Up to 5 µg total DNA can be eluted into ≥ 10 µl. 6 – 1000 ng/ml for healthy females. 2 – 20 ng/ml for healthy males. |
Q1: If I plan to process my urine samples immediately, is the Urine Conditioning Buffer necessary?
The Urine Conditioning Buffer (UCB) is necessary if you are interested in isolating cell-free DNA. If omitting the UCB, only cellular DNA is recovered.
Q2: What is the function of the Clearing Beads?
When the Urine Conditioning Buffer is added to the urine, the Clearing Beads helps to create a compact pellet and make it the pellet easy to visualize during the procedure.
Q3: Can I process urine samples stabilized in DNA/RNA Shield?
Yes, however, the volume of sample processed becomes large and difficult to handle. In addition, cellular and cell-free DNA cannot be separated. To process urine samples stabilized in DNA/RNA Shield, please contact technical support at tech@zymoresearch.com.
Q4: What should I do if I see an oil layer on top of the pellet, after centrifugation?
Remove the oil layer by pipetting and process the pellet. However, residual oil will not affect purification.
Q5: Can cell-free DNA be isolated from urine stored at – 80 °C?
We recommend stabilizing fresh urine with the Urine Conditioning Buffer and storing stabilized urine for later processing. Frozen urine can be processed, however, cells may lyse open upon freeze-thaw, causing in fragmentation of gDNA and resulting in more cell-free DNA than what was originally present.
Q6: Is the <i>Quick</i>-DNA Urine kit compatible with DNA Urine Preserve from Streck?
Yes, however, we cannot guarantee the efficiency of the Streck preservative.
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