Best DNA Extraction Kit For High-Quality DNA

There have been significant developments in the field of molecular biology, and since these techniques are being impacted with several factors, using the best DNA extraction kit that provides efficient DNA extraction has become very important. DNA extractions and purification processes require the best equipment, as any subtle issue can lead to inaccuracy in results.  Automated sequencing is the most commonly-used DNA extraction technique, which is typically used for high-purity DNA. This technique also ensures reduced levels of contaminants.

The DNA-binding medium is the most integral part of DNA extraction kits. This medium should be able to strongly bind DNA on a large surface area in order to offer better yields. Moreover, DNA binding media must also show weak binding to different molecules and proteins so that the RNA and other small molecules can be extracted from the solvent (the binding media) using low-salt buffer conditions.

Best DNA Extraction Kits For Plasmids

Genomic DNA extraction kits should be used for plasmids. When genomic DNA is extracted, all the DNA is taken out, whereas for isolating plasmid DNA, circular plasmid DNA is separated from the bacterial genomic DNA. In addition to that, most part of the isolated plasmid DNA has to be supercoiled for better and improved efficiency in transfections. Transfections are more commonly labelled as downside applications.

There are a plethora of techniques to isolate plasmid DNA. The common DNA extraction kit utilises a type of alkaline lysis, along with incubation with a DNA binding matrix, thorough rinsing, followed by elution. The best DNA purification kit for plasmids offers a profusion of benefits, as it makes use of modified alkaline lysis. These benefits are:

• No harmful substances like phenol or chloroform
• No need for ethanol precipitation
• High-protein media are used for effective purification
• The purification process of high-quality DNA is made fast
• DNA is made ready for downstream applications, cloning, PCT, and cloning as well.
• Every protocol is cost-efficient

The plasmid DNA extraction kit requires a scale. This scale mostly depends on how much initial material is used and also takes into account the yield of plasmid DNA. Generally, these kits come in maxi, mini, and midi prep sizes. Most of the applications require mini kit sizes, as they are best suited for screening. The midi kit isn’t used in most cases, but when the need for producing sufficient plasmid DNA arises, these midi kits are best for several manipulations. Furthermore, high-volume applications like the southern blotting are also done using midi kits.

Use Of DNA Extractions Kits For Genomic And Other DNA

The procedure followed using a DNA extraction kit for genomic DNA majorly relies on the source. All the DNA extraction kits mostly come with alternative cell lysis along with wash protocols that are made to suit the tissue types and common cells.

The environmental samples usually come with several substances that slow down the downstream applications and therefore should be removed. For example, the soil has a lot of humic acids that hinder the process of enzymes including Taq polymerases. This is why the Bacterial genomic DNA extraction kit, which is used for isolating bacterial DNA from soil samples, should be good enough to properly wash the humic acid from the bacterial DNA.