The Quick-DNA/RNA MagBead kit provides a high-throughput, magnetic bead-based purification of both high-quality DNA and total RNA (including small/microRNAs) from the same starting sample. The provided DNA/RNA Shield inactivates infectious agents and is ideal for sample storage at ambient temperatures. The extraction method has the option to recover total nucleic acids in one elution or DNA & RNA in separate elutions without the use of phenol. DNA/RNA is eluted into ≥50 µl of DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing, RT/PCR, hybridization, etc. Order now in USA and Canada from MBP Inc.
| Binding Capacity | 15 µg DNA/RNA per 30 µl MagBinding Beads. |
|---|---|
| Equipment Needed | Magnetic stand or separator, heat block, liquid handler or robotic sample processer (user provided). |
| Purity | High-quality DNA/RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc. |
| Recommended Materials | (sold separately) – 96-well Collection Plate (C2002; capacity is up to 1.2 ml/well), 96-Well Block (P1001; capacity is up to 2 ml/well), 96-well Elution Plate (C2003), Cover Foil (C2007), ZR-96 MagStand (P1005), DNase/RNase-Free Tubes (1.5 ml; C2001). |
| Sample Preservation | DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures (page 7). |
| Sample Sources | Any cells, solid tissue, whole blood, biological fluids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), samples stored in DNA/RNA Shield, etc. |
| Size Limits | Genomic DNA up to and above 40 kb. Mitochondrial and viral DNA (if present) will also be recovered. Total RNA including small/microRNAs ≥17 nt. |
| Storage | DNA and RNA eluted with DNase/RNase-Free Water (provided) can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage. |
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