The D408 Zymo Quick-DNA Magbead Plus DNA Kit in the USA and Canada is the easiest method for high throughput total DNA extraction (e.g., genomic, mitochondrial, viral) from any biological fluid, cell culture, or solid tissue sample. Innovative reagents and Zymo’s unique system allow for a simple Bind, Wash, & Elute procedure that is unmatched in providing ultra-pure and concentrated genomic DNA (up to 150kb) in as little as 60 minutes for 96 samples. Purified DNA is ready for quantification or applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
| Elution Volume | 50 µl DNA Elution Buffer per 33 µl MagBinding Beads |
|---|---|
| Equipment | Magnetic rack, shaker and/or rotator, automated liquid handler (optional) |
| Purity | High quality DNA is ready for all sensitive downstream applications such as long read sequencing, PCR, endonuclease digestion, Southern blotting, genotyping, Next-Generation Sequencing, bisulfite conversion, etc. (A260/A230 ≥ 1.8). |
| Size Range | Capable of recovering genomic and mitochondrial DNA sized fragments up to 150kb. If present, parasitic, microbial, and viral DNA will also be recovered. |
| Workflow | Utilizes a Proteinase K Digestion and magnetic binding bead technology for effective recovery of DNA from blood, cells, and tissues. |
| Yield | The DNA binding capacity is 10 µg per 50 µl MagBinding Beads used. |
| Supplemental Info |
Q1: Can I use more sample input, than what’s recommended in the protocol, by scaling up the protocol?
Custom solutions can be provided, please contact automation@zymoresearch.com.
Q2: Do you have scripts available for your automated kits and/or do you provide scripting support?
Yes, we currently have scripts for Hamilton and KingFisher, as well as support for Tecan on some systems. The kit instruction manual includes an “automation guide” for users to script the protocol themselves and our automation support staff also provides automation troubleshooting and advice if needed. For detailed help regarding all automation questions, please contact automation@zymoresearch.com.
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