The R1059 Zymo Quick-cfRNA Serum & Plasma Kit enables simple, reliable, and efficient preparation of high-quality circulating cell-free RNA (including protein-bound, exosomal, miRNA and other small RNAs) from serum, plasma and other biological fluids. The kit does not use toxic phenol-chloroform or cumbersome protein precipitation and removal steps. Recovered cell-free RNA scales linearly relative to the sample input volume up to 3 ml. ZymoSpinâ„¢ Technology enables the isolation of ultra-pure cell-free RNA suitable for all subsequent analyses, such as RT-qPCR and NGS. Empower your discovery of circulating RNA biomarkers.
Now order R1059 Zymo Quick-cfRNA Serum & Plasma Kit online at the best price in the USA and Canada from MBP Inc.
| Elution Volume | cfRNA can be eluted into 15 µl (to maximize total yield) or as little as 6 µl (for highly concentrated eluate) of DNase/RNase-Free Water. |
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| Processing Time | Typically requires 30 minutes hands-on time to process 10 samples. Sample digestion requires 2 hours. |
| Purity | High quality cell-free RNA (cfRNA) is ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing. |
| Required Equipment | Microcentrifuge, vortex, water bath or heating block, vacuum, and vacuum manifold. |
| Sample Type | Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids. |
| Size | RNA ≥ 17 nt. |
| Yield | Yields are sample dependent and may vary. Typical recovery ranges from 1 – 100 ng/ml of human plasma or serum. Kit is optimized for recovery of total cfRNA, including small RNA and microRNA. |
Q1: Can saliva be used as sample input?
Saliva must be cleared to remove debris/mucus (12,000xg for 15 min at room temperature). Up to 1 ml cleared salvia can be used per extraction.
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