D4076 Zymo Quick-cfDNA Serum & Plasma Kit provides a simple and reliable method for the rapid preparation of high-quality circulating cell-free DNA from serum, plasma, amniotic fluid, cerebrospinal fluid (CSF), and saliva. A combination of chemical and enzymatic methods are used to efficiently recover total DNA (including cell-free apoptotic, necrotic, mitochondrial and viral DNA) linearly from up to 10 ml of sample (Reference Figure A). Zymo-Spin technology allows for ultra-pure DNA to be eluted in as little as 35 μl water. The resulting DNA is suitable for all subsequent analyses and molecular manipulations such as qPCR, Next-Generation sequencing and DNA methylation analyses. Zymo’s serum and plasma cell-free DNA extraction technology will empower your discovery of circulating DNA biomarkers.
|DNA Purity||High-quality DNA is ready for all sensitive downstream applications such as qPCR and Next-Generation sequencing.|
|DNA Recovery||Recover DNA ≥ 100bp (optimized for recovery of cell-free DNA). Yields can vary considerably among different individuals. Typically DNA recovery ranges from 1-100 ng/ml of plasma or serum. The yield varies depending on the sample source and the health of the donor.|
|DNA Storage||Eluted DNA should be stored at ≤ -20ºC|
|Processing volume||Plasma – Single centrifugation: up to 3 ml
Plasma – Double centrifugation: up to 10 ml
Serum – up to 10 ml
Amniotic fluid – up to 1 m
Cerebrospinal fluid (CSF) – up to 1 ml
Saliva – up to 1 ml
Cell-free saliva – up to 5 ml
|Recovery Volume||≥ 35 µl of DNA Elution Buffer or DNase free water.|
|Required Equipment||Water bath or heat block (55ºC), microcentrifuge, vacuum/vacuum manifold or swinging bucket centrifuge.|
|Sample Sources||Serum, plasma, amniotic fluid, and cerebrospinal fluid (CSF).|
Q1: Is the kit compatible to Streck tubes and PAXgene cfDNA tubes?
Yes, both Streck DNA and PAXgene tubes are compatible. First pellet down samples, remove the supernatant, and proceed with the protocol.
Q2: How to process samples stabilized in DNA/RNA Shield with the kit?
This kit is not compatible with samples stored in DNA/RNA Shield.