The Quick-cfDNA/cfRNA Serum & Plasma Kit enables simple and efficient preparation of all circulating cell-free DNA and RNA (including protein-bound, exosomal, microRNA, and other small RNA) from serum, plasma, and other biological fluids, without using toxic phenol-chloroform or cumbersome protein precipitation and removal steps. The kit efficiently separates cell-free DNA and RNA, with option to co-elute. Cell-free nucleic acids scale linearly relative to the sample input volume up to 3 ml. Zymo-Spinā¢ Technology enables isolation of ultra-pure cell-free nucleic acids suitable for all subsequent analyses, such as RT-qPCR and NGS.
| Elution Volume | cfDNA and cfRNA can be eluted into 15 Āµl (to maximize total yield) or as little as 6 Āµl (for highly concentrated eluate) of DNase/RNase-Free Water. |
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| Processing Time | Typically requires 30 minutes (cfDNA/cfRNA co-elution protocol) to 45 minutes (cfDNA/cfRNA separation protocol) hands-on time to process 10 samples. Sample digestion requires 2 hours. |
| Purity | High quality cell-free DNA (cfDNA) and cell-free RNA (cfRNA) are ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing. |
| Required Equipment | Microcentrifuge, vortex, water bath or heating block, vacuum, and vacuum manifold. |
| Sample Types | Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids. |
| Size | DNA ā„ 50 bp and RNA ā„ 17 nt. |
| Yield | Yields are sample dependent and may vary. Typical recovery ranges from 1 ā 100 ng/ml of human plasma or serum for both cfDNA and cfRNA. Kit is optimized for recovery of total cfDNA and cfRNA, including small RNA and microRNA. |
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