|Description||G949 qPCR Retrovirus Titer Kit is a one-step assay which employs a quick RNA extraction step that is followed by RT-qPCR. Designed to deliver high sensitivity and specificity, the kit ensures minimal non-specific background and better overall performance compared to similar kits on the market. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments. MasterMix contains dye comparable to SYBR Green™ and EvaGreen™.|
|Unit quantity||100 rxn|
|Note||qPCR Retrovirus Titer Kit comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.|
|Storage Condition||Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly.|
What is the primer design based on?
The primers in this kit uses a set of proprietary sequences designed based on the 5’LTR region.
How come my cells are not successfully infected with the evaluated titer from the kit?
One important thing to note is that the infection success is dependent on the cell type. Some cells may be more susceptible than others for viral infection. Thus, the same number of infectious units may perform differently on different cell types.