Name: Poly(A) Polymerase, Yeast
SKU: E017
Price: 215.00 USD
Availability: InStock

Poly(A) Polymerase, Yeast

$ 215.00

Description	Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3′ ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5′-triphosphate (3′-dATP) for ATP results in addition of a single dA residue to the 3’-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli.
SKU	E017
Unit quantity	100 U (100 μl)
Applications	• Labelling of RNA with ATP or cordycepin • Poly(A) tailing of RNA for cloning or affinity purification • Increasing translation of RNA transferred into eukaryotic cells
Components	Enzyme supplied with 5X Reaction Buffer.
Concentration	1 U/μl
Storage Condition	Store all components at -20°C.
Storage Buffer	20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton® X-100 and 50% (v/v) Glycerol.
Note	One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C. This product is distributed for laboratory research only. Caution: Not for diagnostic use .

Supporting Protocol

MSDS

Other

T4 Gene 32 Protein

Description	Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3′ ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5′-triphosphate (3′-dATP) for ATP results in addition of a single dA residue to the 3’-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli.
SKU	E017
Unit quantity	100 U (100 μl)
Applications	• Labelling of RNA with ATP or cordycepin • Poly(A) tailing of RNA for cloning or affinity purification • Increasing translation of RNA transferred into eukaryotic cells
Components	Enzyme supplied with 5X Reaction Buffer.
Concentration	1 U/μl
Storage Condition	Store all components at -20°C.
Storage Buffer	20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton® X-100 and 50% (v/v) Glycerol.
Note	One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C. This product is distributed for laboratory research only. Caution: Not for diagnostic use .