R1003 / R1007
Highlights
- High Quality:?Allows for the isolation of total RNA from fresh tissue sections (System I) or FFPE tissues (System II) on glass slides.
- Simple:?Pinpoint tissue sampling technology with a one-step RNA extraction/purification method.
- Concentrated: Up to 10 ?g sample in ? 6 ?l elution.
Description
The R1003 Zymo Pinpoint Slide RNA Isolation Systems I and II are innovative products for the isolation of RNA from any targeted area of fresh, paraffin-fixed, or paraffin-embedded tissues sectioned onto a glass slide. The system combines powerful Pinpoint tissue sampling methodology, a unique single-step RNA extraction/binding buffer, and Zymo-Spin column purification technology to yield high quality RNA. Unlike current UV-based methods, this product makes isolation of tissue RNA simple and quick. No expensive specialized equipment is needed. Eluted RNA is well suited for subsequent RNA analyses including RT-PCR.
Equipment
Microcentrifuge
Purity
RNA is ready for RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source
Cells and tissue sections from fresh, paraffin-fixed, or paraffin-embedded tissue sections on glass slides
Size Range
Total RNA ? 17 nt
Yield
10 ?g RNA (binding capacity), ?6 ?l (elution volume)
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93 / 100 Bioz Stars |
Chromatin accessibility programs CD8 T cell memory
J Immunol Published 15 Mar 2018
‘Cells were fixed in 1% paraformaldehyde and virus-specific P14 cells were sorted by FACS using markers described above.. RNA was prepared using the Pinpoint Slide RNA Isolation System (Zymo Research).. ATAC-seq libraries were generated from 104 cells for each sample, sequenced, and data processed as detailed previously ( ).’
KIF7 attenuates prostate tumor growth through LKB1-mediated AKT inhibiti…
Oncotarget Published 26 Apr 2017
‘Cells were treated with paclitaxel from 0 to 30 nM for 3 days.. FFPE PCa and normal tissue blocks were microdissected for RNA extraction using a Pinpoint? Slide RNA Isolation System II kit (Zymo Research, Orange, CA).. RNA isolation from cells, cDNA synthesis and quantitative real-time polymerase chain reaction (qPCR) were performed as described [ ].’
A PGC1?-mediated transcriptional axis suppresses melanoma metastasis
’18S rRNA was used as internal control.. For cells from paraffin-embedded tissue sections, Pinpoint? Slide RNA Isolation System II (Zymo Research) was used to extract RNAs.. Cells with different mitochondrial contents were sorted based on the labeling of MitoTracker Green (Invitrogen).’
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