Highlights
- Pellet-Free: Direct lysis procedure omits cell-pelleting, resuspension steps, and large centrifuges.
- Quick & amp; Pure: 15 minutes from culture flask to transfection-grade plasmid DNA.
- Highest Yield: Purify up to 1.2 mg of plasmid DNA using a spin-column.
Description
The ZymoPURE plasmid purification kits are the best method for rapid isolation of transfection ready plasmid DNA. Plasmid purification for Mini preps, Midi preps and Maxi preps is performed in less than 20 minutes and Giga preps in 50 minutes. This family of plasmid purification kits feature a patented binding chemistry that enables simple purification of highly-concentrated (up to 3 mg/ml) endotoxin-free plasmid DNA on a spin-column. The streamlined workflow eliminates slow gravity flow anion-exchange columns and isopropanol precipitation steps found in other kits. ZymoPURE plasmid purification kits reduce processing time by up to 7x using a vacuum manifold or centrifugation. Simply bind, wash and elute transfection ready, endo-free plasmid in minutes.
The ZymoPURE plasmid purification kits are optimized to ensure the eluted DNA is free of endotoxins, salt, protein, and RNA, resulting in plasmids that are suitable for use in sensitive applications. ZymoPURE II plasmid purification kits include the EndoZero columns that enable rapid isolation of endotoxin-free plasmid DNA. The resulting endo-free plasmid DNA is ideal for transfection1 (including sensitive and primary cells), CRISPR-Cas9 and gene editing2, lentiviral vectors3, adenovirus vectors3 and AAV vectors3, gene therapy, chimeric antigen receptor (CAR) generation4, recombinant antibody generation5, in vitro transcription6, synthetic biology7, PCR8, transgenic organism generation9 and microinjections10, molecular cloning11, restriction endonuclease digestions, site-directed mutagenesis12, plasmid transformation of competent cells, Sanger sequencing13, and other sensitive downstream applications.
Technical Specifications
| Applicable For | Eluted DNA is well suited for transfection, sequencing, cloning, plasmid mediated gene silencing, in vitro transcription, and other sensitive applications. |
|---|---|
| Binding Capacity | 1.2 mg |
| Culture Input | ≤ 25 mL |
| Elution Volume | ≥ 200 µl |
| Endotoxin Levels | ≤1 EU/µg Plasmid DNA |
| Equipment | Microcentrifuge and vacuum/vacuum manifold |
| Format | Spin Column |
| Processing Time | 15 min |
| Purity | Typical Abs260/280 ≥1.8 and Abs260/230≥ 2.0 |
| Recommended Sample Volume | ≤ 25 ml |
| Size Range | Up to 200 kb |
| Yield | ≤ 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized). 1.2 mg maximum binding capacity of column. |
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