- Pellet-Free: Rapid direct lysis procedure omits cell-pelleting and resuspension steps
- High Quality: Ready for PCR, sequencing, cloning, and transfection
- Ideal for Synthetic Biology: Fastest, high-throughput automated method for preparing high-quality plasmid DNA.
The Zyppy-96 Plasmid MagBead Miniprep is the fastest high-throughput (96-well), Pellet-Free plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are expedited using a specially designed Neutralization Buffer. Plasmid DNA is then purified using the featured magnetic bead based technology. This straightforward procedure is compatible for either automated or manual processing. Eluted plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection. An overview of the purification procedure is shown below.
|Applicable For||Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.|
|Elution Volume||≥ 30 µl|
|Equipment||Magnetic Stand, Heating Element, or Liquid Handler|
|Processing Time||60 min|
|Purity||Typical Abs 260/280 ≥1.8.|
|Size Range||Up to 25 kb|
|Yield||Up to 5 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli processed.|