Highlights
- Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
- High Quality: Ready for PCR, sequencing, cloning, and transfection.
- Streamlined Protocol: Culture, lyse, and neutralize in the same plate, saving time and reducing plastic waste.
Description
The Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.
Technical Specifications
| Applicable For | Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. |
|---|---|
| Elution Volume | ≥ 30 µl |
| Equipment | Centrifuge with micro plate carriers |
| Processing Time | 45 min |
| Purity | Typical Abs 260/280 ≥ 1.8. |
| Size Range | Up to 25 kb |
| Yield | Up to 5 µg per preparation depending on the plasmid copy number, culture growth conditions, and strain of E. coli processed. |
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