OneScript® Plus Reverse Transcriptase is a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C – 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates.
OneScript® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor (Cat. No. G138) offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.
Product Features:
- High thermostability at 50-55°C/122-131°F
- Synthesize 15 kb RNA Templates
- 10-15 minutes reaction time
- Earn 2X the rewards points
- OneScript® Plus cDNA Synthesis Kit (Cat. No. G236) also available
- Pair with BlasTaq™ 2X PCR MasterMix (Cat. No. G895) for Two-Step RT-PCR workflows
Supporting Protocol
MSDS
QC
Other
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