OneScript® Plus cDNA Synthesis Kit contains a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C – 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. OneScript® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.
Key Features
- Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers
- Robust cDNA synthesis from any RNA template
- High reproducibility and excellent yield
- Employs abm‘s OneScript® Plus Reverse Transcriptase (Cat. No. G237)
- Earn 2X the rewards points
- Supporting Protocol
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