Specifications
| SKU | E057 |
|---|---|
| Unit quantity | 10000 U (500 µl) |
| Applications | Restriction enzyme digestion of double-stranded DNA |
| Components | MfeI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) |
| Concentration | 20000 U/ml |
| Expression System | An E.coli strain that carries the modified MfeI gene from Mycoplasma fermentas (N.F. Halden). The MfeI enzyme recognizes and cuts the sequence C^AATTG. |
| Inactivation Protocol | No |
| Reaction Definition | 1X Universal Restriction Enzyme Reaction Buffer (Cat. No. E204). Incubate at 37°C |
| Reaction Buffer | Buffer 1: 75%, Buffer 2: 25%, Buffer 3: <10%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes. |
| Storage Condition | -20°C |
| Storage Buffer | 10mM Tris pH (7.5), 100mM NaCl, 1mM DTT, 0.1mM EDTA, and 50% (v/v) Glycerol. |
| Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked by Some Combinations of Overlapping |
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