Specifications
Description | KIJ265T Cells are derived from a sample of primary tumor site stage IV human ccRCC patient tissue. |
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SKU | T8249 |
Species | Human (H. sapiens) |
Tissue/Organ/Organ System | Kidney |
Donor Gender | Female |
Donor Disease | Clear cell renal cell carcinoma |
Growth Properties | Adherent |
Cell Morphology | Polygonal |
Applications | For Research Use Only |
Unit quantity | 1×106 cells / 1.0 ml |
Cell Type | Tumor Cells |
Propagation Requirements |
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below. The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 5%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, and HEPES to a final concentration of 10 mM. |
Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase. |
QC | 1) STR profiling |
Disclaimer |
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Depositor | Mayo |
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- Hitora, T., Yamamoto, T., Akisue, T., Marui, T., Nakatani, T., Kawamoto, T., … & Kurosaka, M. (2005). Establishment and characterization of a KIT‐positive and stem cell factor‐producing cell line, KTHOS, derived from human osteosarcoma. Pathology international, 55(2), 41-47.
- Tun HW, Marlow LA, von Roemeling CA, Cooper SJ, Kreinest P, Wu K, Luxon BA, Sinha M, Anastasiadis PZ, Copland JA. Pathway signature and cellular differentiation in clear cell renal cell carcinoma. PLoS One. 2010 May 18;5(5):e10696. doi: 10.1371/journal.pone.0010696. PMID: 20502531; PMCID: PMC2872663.
- Cooper SJ, von Roemeling CA, Kang KH, Marlow LA, Grebe SK, Menefee ME, Tun HW, Colon-Otero G, Perez EA, Copland JA. Reexpression of tumor suppressor, sFRP1, leads to antitumor synergy of combined HDAC and methyltransferase inhibitors in chemoresistant cancers. Mol Cancer Ther. 2012 Oct;11(10):2105-15. doi: 10.1158/1535-7163.MCT-11-0873. Epub 2012 Jul 23. PMID: 22826467; PMCID: PMC3928542.
- von Roemeling CA, Marlow LA, Wei JJ, Cooper SJ, Caulfield TR, Wu K, Tan WW, Tun HW, Copland JA. Stearoyl-CoA desaturase 1 is a novel molecular therapeutic target for clear cell renal cell carcinoma. Clin Cancer Res. 2013 May 1;19(9):2368-80. doi: 10.1158/1078-0432.CCR-12-3249. Epub 2013 Apr 30. PMID: 23633458; PMCID: PMC3644999.