|Description||KBDC-11 Cells are isolated from a pancreatic head and perihilar cholangiocarcinoma patient with intraductal papillary neoplasm of the bile duct (IPNB). KBDC-11 Cells retained characteristics similar to those of the primary tumor such as overexrpression of p53, positivity for MUC1 and MUC5, and negativity for MUC2 and CD133. The cells require a mouse feeder layer of PA6 cells to be grown to form colonies. Cell grafts of KBDC-11 Cells fomed subcutaneous tumor on injection site, but metastatic tumors were not detected. A valuable model for IPNB-related research such as carcinogenesis, and moleular biological mechanisms.|
|Species||Human (H. sapiens)|
|Donor Ethnicity||Donor Info Not Disclosed|
|Donor Disease||Intraductal papillary neoplasm of the bile duct (IPNB)|
|Applications||For Research Use Only|
|Unit quantity||1×106 cells/1.0ml|
|Cell Type||Tumor Cells|
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The cells must be grown on top of a feeder layer of PA6 cells (RIKEN). The base medium for this cell line is serum-free Stem medium (DS Pharma Biomedical). To make complete media add a final concentration of 0.1 ?M 2-mercaptoethanol and Penicillin/Streptomycin Solutiom (G255) to a final concentration of 1%.
|QC||1) Staining with hematoxylin and eosin, Alcian blue, and for p53 and CD133|
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3. All test parameters provided in the CoA are conducted using abm‘s standardized culture system and procedures. The stated values may vary under the end-user’s culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
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|Caution||For Research Use Only|
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- Hitora, T., Yamamoto, T., Akisue, T., Marui, T., Nakatani, T., Kawamoto, T., … & Kurosaka, M. (2005). Establishment and characterization of a KIT‐positive and stem cell factor‐producing cell line, KTHOS, derived from human osteosarcoma. Pathology international, 55(2), 41-47.
- Murakami, S., Ajiki, T., Hori, Y., Okazaki, T., Fukumoto, T., & Ku, Y. (2014). Establishment of a Novel Cell Line from Intraductal Papillary Neoplasm of the Bile Duct. Establishment Of A Novel Cell Line From Intraductal Papillary Neoplasm Of The Bile Duct, 34(5), 2203-2209.