Specifications
| Description | KBDC-11 Cells are isolated from a pancreatic head and perihilar cholangiocarcinoma patient with intraductal papillary neoplasm of the bile duct (IPNB). KBDC-11 Cells retained characteristics similar to those of the primary tumor such as overexrpression of p53, positivity for MUC1 and MUC5, and negativity for MUC2 and CD133. The cells require a mouse feeder layer of PA6 cells to be grown to form colonies. Cell grafts of KBDC-11 Cells fomed subcutaneous tumor on injection site, but metastatic tumors were not detected. A valuable model for IPNB-related research such as carcinogenesis, and moleular biological mechanisms. |
|---|---|
| SKU | T8200 |
| Species | Human (H. sapiens) |
| Tissue/Organ/Organ System | Digestive |
| Donor Gender | Male |
| Donor Gender | Male |
| Donor Ethnicity | Donor Info Not Disclosed |
| Donor Age | 69 |
| Donor Disease | Intraductal papillary neoplasm of the bile duct (IPNB) |
| Growth Properties | Adherent |
| Cell Morphology | Spherical |
| Applications | For Research Use Only |
| Unit quantity | 1×106 cells/1.0ml |
| Cell Type | Tumor Cells |
| Propagation Requirements |
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The cells must be grown on top of a feeder layer of PA6 cells (RIKEN). The base medium for this cell line is serum-free Stem medium (DS Pharma Biomedical). To make complete media add a final concentration of 0.1 ?M 2-mercaptoethanol and Penicillin/Streptomycin Solutiom (G255) to a final concentration of 1%. |
| QC | 1) Staining with hematoxylin and eosin, Alcian blue, and for p53 and CD133 |
| Disclaimer |
1. For for-profit organizations and corporations, please contact quotes@abmgood.com for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at licensing@abmgood.com. 3. All test parameters provided in the CoA are conducted using abm‘s standardized culture system and procedures. The stated values may vary under the end-user’s culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination’s temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm‘s cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the “Warranty Period.” |
| Caution | For Research Use Only |
| Depositor | Kobe University |
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- Hitora, T., Yamamoto, T., Akisue, T., Marui, T., Nakatani, T., Kawamoto, T., … & Kurosaka, M. (2005). Establishment and characterization of a KIT‐positive and stem cell factor‐producing cell line, KTHOS, derived from human osteosarcoma. Pathology international, 55(2), 41-47.
- Murakami, S., Ajiki, T., Hori, Y., Okazaki, T., Fukumoto, T., & Ku, Y. (2014). Establishment of a Novel Cell Line from Intraductal Papillary Neoplasm of the Bile Duct. Establishment Of A Novel Cell Line From Intraductal Papillary Neoplasm Of The Bile Duct, 34(5), 2203-2209.
