|Description||Clonal cell line isolated from NCIH295R. The HAC50 cells increase aldosterone production in response to treatment with angiotensin II and high extracellular potassium levels. These cells respond to activation cAMP signaling pathway agonists, forskolin and dibutyryl cAMP, with a time-dependent increase in cortisol and dehydroepiandrosterone production. In summary, the HAC50 cell line is capable of responding to the three main adrenocortical physiologic regulators.|
|Species||Human (H. sapiens)|
|Tissue/Organ/Organ System||Adrenal gland|
|Donor Age||48 years|
|Donor Disease||Adrenal carcinoma|
|Applications||For Research Use Only|
|Unit quantity||1×106 cells / 1.0 ml|
|Cell Type||Tumor Cells|
|Expression Profile||Amelogenin: X|
|Propagation Requirements||Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow IV (TM004) + 10% cosmic calf serum + 1X ITS Plus + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Selection with 1 µg/ml G418 (G271).|
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2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at email@example.com.
3. All test parameters provided in the CoA are conducted using abm‘s standardized culture system and procedures. The stated values may vary under the end-user’s culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination’s temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
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6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the “Warranty Period.”
|Depositor||Augusta University & The University of Texas Southwestern Medical Center|
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- Wang, T., & Rainey, W. E. (2012). Human adrenocortical carcinoma cell lines. Molecular and cellular endocrinology, 351(1), 58–65. https://doi.org/10.1016/j.mce.2011.08.041
- Wang, T., Rowland, J. G., Parmar, J., Nesterova, M., Seki, T., & Rainey, W. E. (2012). Comparison of aldosterone production among human adrenocortical cell lines. Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme, 44(3), 245–250. https://doi.org/10.1055/s-0031-1298019
- Nanba, K., Blinder, A. R., & Rainey, W. E. (2021). Primary Cultures and Cell Lines for In Vitro Modeling of the Human Adrenal Cortex. The Tohoku journal of experimental medicine, 253(4), 217–232. https://doi.org/10.1620/tjem.253.217
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