|Description||The HT-29-C1.16E cells were derived from the human colonic cancer cell line HT29. This cell line has 600 times more mucus than the original HT29 cells. The HT-29-C1.16E cells can be used for investigation of intestinal cell differentiation.|
|Species||Human (H. sapiens)|
|Applications||For Research Use Only|
|Cell Type||Drug Discovery Cell Lines|
|Propagation Requirements||Use of Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum (TM999)* to a final concentration of 10% and Pen/Strep Solution (G255) to a final concentration of 1%.
|Unit quantity||1×106 cells / 1.0 ml|
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- Laboisse CL, Augeron C, Couturier-Turpin MH, Gespach C, Cheret AM, & Potet F. (1982) Characterization of a newly established human gastric cancer cell line HGT-1 bearing histamine H2-receptors. Cancer Res. (42)(4):1541-1548
- Augeron C, & Laboisse CL.(1984) Emergence of permanently differentiated cell clones in a human colonic cancer cell line in culture after treatment with sodium butyrate. Cancer Res. (44)(9):3961-3969
- Laboisse, C. L., Maoret, J. Triadou, N., & Augeron, C. (1988). Restoration by Polyethylene Glycol of Characteristics of Intestinal Differentiation in Subpopulations of the Human Colonic Adenocarcinoma Cell Line HT29. Cancer Res, (48) (9) 2498-2504.