Name: HighPrep™ Plant DNA Plus Kit
SKU: HPPP-D
Availability: InStock

HighPrep™ Plant DNA Plus Kit

$ 22.50 – $ 1,088.88

APPLICATIONS

Plant genomic DNA isolation for:

PCR, Real-time PCR
Cloning, genotyping
Sequencing
Plant breeding

BENEFITS

Purified PCR grade DNA with no inhibiting substances (polysaccharides and phenols)
Includes beads to homogenize sample
Eliminate need of grinding
Adaptable to automation

Details

The HighPrep™ Plant DNA Plus Kit is specially designed for purifying DNA from a wide range of plant and fungi species. The kit uses the special lysis condition with HighPrep™ magnetic particles technology to isolate the high-quality genomic DNA. Under DNA binding conditions only the genomic DNA will bind to the magnetic particles while most of the contaminating cellular proteinaceous components are removed. The purifi ed DNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, Southern blotting, SNP analysis and NGS etc. The HighPrep™ Plant DNA Plus Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.

Technical Information

Kit Components

PLB Buffer
PDB Buffer
PDW Buffer
DWB Buffer
Elution Buffer
MAG-D1 Particles
Pro K Solution
RNase A

Storage

MAG-R5 Particles should be stored at 2-8°C.
DNase I should be stored at -20°C.
All other components stored at 15-25°C.

Stability

14 months when kit components are stored as directed.

Disclaimers

For Research Use Only. Not for Use in Diagnostic Procedures.

This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS

Plant samples are disrupted in a homogenizer/bead based milling equipment.
PLB Buffer is added to lyse the sample.
Supernatant is then transferred to a new processing plate where MAG-D1 particles are added to bind to the DNA.
Following a few wash steps, DNA is eluted from the MAG-D1 particles for downstream application.