The HighPrep™ DTR Clean-up System is a high performance paramagnetic bead-based reagent for BigDye™ terminator removal from Sanger sequencing reactions for reliable and long readings of DNA sequences. The HighPrep™ DTR Clean-up System consists of a selective binding of DNA to the paramagnetic beads, followed with washing off nucleotides, primers, and non-targeted amplicons, and finally elution of pure DNA. HighPrep™ DTR Clean-up System is an excellent choice for both manual and fully automated purification of sequencing reaction products. The protocol can be adapted to common liquid handling workstation.
Kit Components
- HighPrep™ DTR Clean-up reagent
Storage
Store at 4°C to 8°C | DO NOT FREEZE
Stability
14 months when stored as directed.
Disclaimers
For Research Use Only. Not for Use in Diagnostic Procedures.
Number of reactions
| In 96 Well Format | |
| PCR Reaction Volume | Number of reactions |
| 5 µL | 500 |
| 10 µL | 500 |
| 15 µL | 500 |
| 20 µL | 500 |
| In 384 Well Format | |
| PCR Reaction Volume | Number of reactions |
| 5 µL | 1000 |
| 10 µL | 1000 |
| 15 µL | 1000 |
This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
- Add HighPrep™DTR reagent to sample
- Add freshly prepared 85% ethanol
- Bind sample to paramagnetic beads
- Wash beads with 85% ethanol to remove contaminants
- Elute purified DNA from beads
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