Specifications
| Description | The HGT-1 cell line was derived from a primary gastric adenocarcinoma tumor removed from a 60 year old Moroccan male of O Rh – blood group. At the time of the operation, the primary tumor showed no signs of metastasis but did exhibit poor differentiation. HGT-1 cells show hyperdiploid karyotype with a modal number of 57 chromosomes and are shown to be tumorgenic in nude mice. They also display functional histamine H 2 -receptors mediating cellular cyclic adenosine 3′:5′-monophosphate production and adenylate cyclase activation. HGT-1 cells may have applications in the in vitro study of stomach cancer helping to identify the gene drives associated with gastric cancers. |
|---|---|
| SKU | T8210 |
| Species | Human (H. sapiens) |
| Tissue/Organ/Organ System | Stomach |
| Donor Gender | Male |
| Donor Gender | Male |
| Donor Ethnicity | Moroccan |
| Donor Age | 60 |
| Donor Disease | Gastric adenocarcinoma |
| Growth Properties | Adherent |
| Cell Morphology | Epithelial-like, Polygonal |
| Population Doubling Time | 19 hours |
| Applications | For Research Use Only |
| Unit quantity | 1×106 cells/1.0ml |
| Cell Type | Tumor Cells |
| Propagation Requirements | Grow cells in T25 ECM-coated flasks (G299) with the following conditions. The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the completed growth medium, add the following components to the base medium: heat inactivated-fetal bovine serum to final concentration of 10% and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO2: 5%; Temperature: 37.0°C. Recommend cells be detached from cell culture vessels using a solution of 0.25% Trypsin and 0.02% EDTA. |
| QC | 1) Calculated the growth curve of HGT-1 cells at passage 40 (Chart 1). 2) Observed morphology of HGT-1 cells at passage 70 (Figure 2). 3) Analyzed morphology of the primary tumor through microscopy (Figure 4). 4) Characterized the chromosomal markers and defects HGT-cells karyotype. (Table 1). 5) Tested the production of cyclic AMP and adenylate cyclase activity after the introduction of histamine alone or with cimetidine (Table 2) |
| Disclaimer |
1. For for-profit organizations and corporations, please contact quotes@abmgood.com for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at licensing@abmgood.com. 3. All test parameters provided in the CoA are conducted using abm‘s standardized culture system and procedures. The stated values may vary under the end-user’s culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination’s temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm‘s cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the “Warranty Period.” |
| Caution | For Research Use Only |
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- Hitora, T., Yamamoto, T., Akisue, T., Marui, T., Nakatani, T., Kawamoto, T., … & Kurosaka, M. (2005). Establishment and characterization of a KIT‐positive and stem cell factor‐producing cell line, KTHOS, derived from human osteosarcoma. Pathology international, 55(2), 41-47.
- Laboisse CL, Augeron C, Couturier-Turpin MH, Gespach C, Cheret AM, & Potet F. (1982) Characterization of a newly established human gastric cancer cell line HGT-1 bearing histamine H2-receptors. Cancer Res. (42)(4):1541-1548
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- Laboisse, C. L., Maoret, J. Triadou, N., & Augeron, C. (1988). Restoration by Polyethylene Glycol of Characteristics of Intestinal Differentiation in Subpopulations of the Human Colonic Adenocarcinoma Cell Line HT29. Cancer Res, (48) (9) 2498-2504.
