Name: HaeII
SKU: E060
Price: 115.00 USD
Availability: InStock

HaeII

$ 115.00

SKU	E060
Unit quantity	10000 U (500 µl)
Applications	Restriction enzyme digestion of double-stranded DNA
Components	HaeII enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)
Concentration	20000 U/ml
Expression System	An E. coli strain that carries the HaeII gene from Haemophilus aegypticus (ATCC 11116). The HaeII enzyme recognizes and cuts the sequence RGCGC^Y.
Inactivation Protocol	80°C for 20 min
Reaction Definition	1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204). Incubate at 37°C
Reaction Buffer	Buffer 1: 25%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%. Click here to view the buffer compatibility chart for restriction enzymes.
Storage Condition	-20°C
Storage Buffer	10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, and 50% (v/v) Glycerol.
Note	Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked

Supporting Protocol

MSDS

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EcoRV $ 45.00

HaeIII $ 205.00

SKU	E060
Unit quantity	10000 U (500 µl)
Applications	Restriction enzyme digestion of double-stranded DNA
Components	HaeII enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)
Concentration	20000 U/ml
Expression System	An E. coli strain that carries the HaeII gene from Haemophilus aegypticus (ATCC 11116). The HaeII enzyme recognizes and cuts the sequence RGCGC^Y.
Inactivation Protocol	80°C for 20 min
Reaction Definition	1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204). Incubate at 37°C
Reaction Buffer	Buffer 1: 25%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%. Click here to view the buffer compatibility chart for restriction enzymes.
Storage Condition	-20°C
Storage Buffer	10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, and 50% (v/v) Glycerol.
Note	Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked