Highlights
- Quick, 5-minute clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations.
- Unique spin column for low volume elution of ultra-pure, high-yield DNA.
- Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.
Description
The D4011 Zymo Genomic DNA Clean & Concentrator-10 (gDCC) is a gDNA clean up kit that provides quick, 5 minute recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, WGA DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
Packaging Size: 100 Preps
Applicable For
Eluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream application
Device Specs
2 mL screwcap tube pre-filled with DNA/RNA Shield (1 mL) and ultra-high density BashingBeads (R1103 – 0.5 mm & 0.1 mm beads, R1105 – 2.0 mm beads) for homogenization. R1104 includes swab with 20 mm breakpoint.
Elution Volume
? 10 ?l of DNA Elution Buffer
Elution Volume
10% (v/v or w/v)
Equipment
Microcentrifuge
Purity
A260/A280 > 1.8, A260/A230 > 1.8
Sample Source
Enzymatic reactions or impure preparations containing genomic DNA.
Sample Storage
Eluted DNA can be used immediately or stored at ? -20?C.
Size Range
50 bp to 200 kb
Yield
Recovery of DNA ranges from 70 – 95%
Q1: What is the lower limit and minimal amount of DNA that can be recovered?
Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.
Q2: How can I process naked DNA stored in DNA/RNA Shield?
Use the standard protocol (add 2 or 5 volumes of DNA binding buffer depending on DNA size).
Q3: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?
The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.
Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.
Q5: How many times can columns be reloaded?
Zymo Research recommends no more than 5 times, as binding efficiency might decrease.
Q6: A white precipitate occurred after thawing frozen samples stored in DNA/RNA Shield, is this normal?
Some components of the reagent (or sample) may have precipitated out of solution during the freeze-thaw process. Once the sample is thawed to ambient temperature, vortex the sample to bring precipitate back into solution. Furthermore, try heating samples in hand or 37?C for 5 minutes and vortex.
Q7: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
No, tissues do not need to be homogenized.
Q8: What is the minimum input volume of DNA sample?
Working with volumes below 50 ?l can result in decreased recovery. Zymo Research recommends raising the starting volume to 100??l with water to ensure optimal binding conditions.
 |
99 / 100 Bioz Stars |
Defective IgA response to atypical intestinal commensals in IL-21 recept…
Mucosal Immunol – Published 07 Feb 2019
‘Bacterial DNA was extracted using QIAamp DNA stool kit (Qiagen) and further purified using Genomic DNA clean & Concentrator (Zymo Research).. Isolated DNA was subjected to 16S rRNA gene profiling with Illumina library targeting the V3-V4 region sequenced using a miSeq sequencer.’
The complex architecture and epigenomic impact of plant T-DNA insertions
PLoS Genet – Published 18 Jan 2019
The genomic DNA samples were purified with the Zymogen Genomic DNA Clean and Concentrator-10 column (Zymo Research, Irvine).. The purified DNA was prepared for sequencing with the Ligation Sequencing Kit 1D (SQK-LSK108, ONT, Oxford, UK) sequencing kit protocol.
Defective IgA response to atypical intestinal commensals in IL-21 recept…s
Mucosal Immunol – Published 07 Feb 2019
?Ct = CtSFB/Helicobacter ? CtEubacteria .. To compare SFB levels in the terminal ileum, 5 cm of tissue was homogenized after removing the luminal contents and DNA was purified using QIAamp Fast DNA Stool Kit and Genomic DNA clean & Concentrator (Zymo Research).. Again, melting curve analyses were performed to ensure a single product.
| Cat # | Name | Size |
|---|---|---|
| D3004-4-1 | DNA Elution Buffer | 1 ml |
| D3004-4-4 | DNA Elution Buffer | 4 ml |
| D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml |
| D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml |
| D5201-1-50 | ChIP DNA Binding Buffer | 50 ml |
| C1001-50 | Collection Tubes | 50 Pack |
| C1001-500 | Collection Tubes | 500 Pack |
| C1001-1000 | Collection Tubes | 1000 Pack |
| C1002-25 | Zymo-Spin IC-XL | 25 Pack |
| C1002-50 | Zymo-Spin IC-XL | 50 Pack |
Reviews
There are no reviews yet.