Q1: What is the lower limit and minimal amount of DNA that can be recovered?
Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.
Q2: How can I process naked DNA stored in DNA/RNA Shield?
Use the standard protocol (add 2 or 5 volumes of DNA binding buffer depending on DNA size).
Q3: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?
The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.
Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.
Q5: How many times can columns be reloaded?
Zymo Research recommends no more than 5 times, as binding efficiency might decrease.
Q6: A white precipitate occurred after thawing frozen samples stored in DNA/RNA Shield, is this normal?
Some components of the reagent (or sample) may have precipitated out of solution during the freeze-thaw process. Once the sample is thawed to ambient temperature, vortex the sample to bring precipitate back into solution. Furthermore, try heating samples in hand or 37?C for 5 minutes and vortex.
Q7: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
No, tissues do not need to be homogenized.
Q8: What is the minimum input volume of DNA sample?
Working with volumes below 50 ?l can result in decreased recovery. Zymo Research recommends raising the starting volume to 100??l with water to ensure optimal binding conditions.
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Defective IgA response to atypical intestinal commensals in IL-21 recept…
‘Bacterial DNA was extracted using QIAamp DNA stool kit (Qiagen) and further purified using Genomic DNA clean & Concentrator (Zymo Research).. Isolated DNA was subjected to 16S rRNA gene profiling with Illumina library targeting the V3-V4 region sequenced using a miSeq sequencer.’
The complex architecture and epigenomic impact of plant T-DNA insertions
The genomic DNA samples were purified with the Zymogen Genomic DNA Clean and Concentrator-10 column (Zymo Research, Irvine).. The purified DNA was prepared for sequencing with the Ligation Sequencing Kit 1D (SQK-LSK108, ONT, Oxford, UK) sequencing kit protocol.
Defective IgA response to atypical intestinal commensals in IL-21 recept…s
?Ct = CtSFB/Helicobacter ? CtEubacteria .. To compare SFB levels in the terminal ileum, 5 cm of tissue was homogenized after removing the luminal contents and DNA was purified using QIAamp Fast DNA Stool Kit and Genomic DNA clean & Concentrator (Zymo Research).. Again, melting curve analyses were performed to ensure a single product.
|D3004-4-1||DNA Elution Buffer||1 ml|
|D3004-4-4||DNA Elution Buffer||4 ml|
|D4003-2-24||DNA Wash Buffer (Concentrate)||24 ml|
|D4003-2-6||DNA Wash Buffer (Concentrate)||6 ml|
|D5201-1-50||ChIP DNA Binding Buffer||50 ml|
|C1001-50||Collection Tubes||50 Pack|
|C1001-500||Collection Tubes||500 Pack|
|C1001-1000||Collection Tubes||1000 Pack|
|C1002-25||Zymo-Spin IC-XL||25 Pack|
|C1002-50||Zymo-Spin IC-XL||50 Pack|