E. coli CRISPR Knockout Teaching Kit

SKU: K099 Category: Enzymes

Description	The E. coli CRISPR Knockout Teaching Kit allows students to gain hands-on laboratory experience utilizing CRISPR – a revolutionary gene-editing technique. In this robust experiment, the student performs a gene knockout of a genomically-encoded chloramphenicol-resistance cassette employing CRISPR and lambda red recombineering (see our Knockout Case Study for details). This results in E. coli that is now sensitive to the antibiotic chloramphenicol – a phenotype that can be easily screened for. The experiment is carried out in a safe laboratory strain of E. coli (BSL-1) and uses standard microbiological/molecular biology reagents and equipment allowing for ease of implementation in most teaching labs. For more information, please contact technical@abmgood.com.

Unit quantity	15 Reactions
Applications	Students gain hands-on laboratory experience using the CRISPR gene-editing technique. Experiment performed in E. coli Simple and robust editing occurs in 4 days Uses standard reagents and equipment
Note	This kit is intended for teaching purposes only.