R2054 / R2055 / R2056 / R2057
Highlights
- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Non-Biased: Complete RNA recovery without miRNA loss.
Description:
The R2102 Direct-zol-96 RNA Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 g (per well) of high-quality RNA directly from samples in TRI Reagent. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product. The extraction method inactivates viruses and other infectious agents. The procedure is easy: simply apply a sample in TRI Reagent? to the Zymo-Spin I-96 Plate, then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc. The entire procedure typically takes about 30 minutes (per 2 plates).
Compatibility
TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
Equipment
Microplate centrifuge, vortex
Sample Inactivation
TRI Reagent? (provided with R2055, R2057) inhibits RNase activity and inactivates viruses and other infectious agents.
Sample Source
Any sample stored and preserved in TRI Reagent?, TRIzol? or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA).
Size Range
Total RNA ≥ 17 nt
Yield
10 µg RNA (binding capacity), ≥ 30 µl (elution volume)
Q1: Is DNase I available for individual purchase?
All kit components are available for purchase separately.
Q2: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q3: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q4: Is the kit compatible with samples stored in DNA/RNA Shield?
Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30?C). Add 3 volume of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.
Q5: Is Direct-zol suitable for very small numbers of cells?
Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)
Q6: Is it possible to extract proteins with the Direct-zol RNA kits?
Yes, proteins can be Acetone Precipitated post RNA binding step. Please request supplementary protocol from Zymo Research Technical Support.
Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?
Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?
Direct-zol DNA/RNA (D2080) kits can isolate DNA from TRIzol.
Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?
Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.
Q10: Which phenol-based reagents are compatible with Direct-zol?
Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?
Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.
Q13: I ran out of RNA Wash Buffer. Can I use something else?
Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
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99 / 100 Bioz Stars |
Epstein?Barr virus-encoded EBNA2 alters immune checkpoint PD-L1 expressi…
Leukemia Published 26 Jun 2019
‘PD-L1 (E1L3N, cat# 13684) and p21 (#2947) and BCL2 (#15071) were purchased from Cell Signaling.. Total RNA from cell lines was isolated using Direct-zol RNA MiniPrep Plus kit (Zymo Research) according to the vending company?s instructions.. The integrity of RNA was routinely checked using 1% agarose gel and RNA quantification was estimated with a DS-11 spectrophotometer (DeNovix) [ ].’
Optimizing PCR Detection of Zika Virus from Various Body Fluids
The American Journal of Tropical Medicine an… Published 21 Feb 2019
‘?80?C freezing steps were tested: 1) right after spiking (then UCB added while still frozen and thawed with constant mixing); and 2) freezing the UCB pellet. . Additional procedures and reagents/supplies were tested to improve viral RNA recovery from semen and urine samples, including buffers and columns of RNeasy Plus Mini Kit or QIAamp Viral RNA Mini Kit, QIAzol, QIAshredder columns, ATL buffer (all QIAGEN), Direct-zol RNA MiniPrep Kit (Zymo Research), RNasin Plus RNase Inhibitor (Promega, Madison, WI), SUPERase In RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA), Protector RNase Inhibitor (Roche, Indianapolis, IN), ProtectRNA RNase Inhibitor (Sigma-Aldrich, St. Louis, MO), and Aptima Urine Specimen Transport Tubes (Hologic, Inc., Marlborough, MA).. Five microliters of extracted RNA were used in a duplicate 20-?L reaction with TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) on ViiA ‘
Diseases caused by different mutations in the two alleles of a gene are …
Nat Biotechnol Published 13 Feb 2019
‘The FAH antibody used in IHC (Abcam, Cat. No. ab81087) was 1:400 diluted.. An aliquot of tissue powder was used to extract total RNA using the Direct-zol RNA Miniprep kit (Zymo Research, Cat. No. R2052).. RNA was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Fisher Scientific, Cat. No. 43-688-13), and subjected to PCR with ‘
?Before I discovered this kit, I was isolating RNA the old school way with chloroform and it would take half the day to finish the protocol. The Direct-zol RNA Miniprep kit is AWESOME! It took hardly any time, the protocol was so easy, and my RNA quality was SO much better. Honestly, this kit revolutionized my life at the bench.?
– Umberto P. (University of Pavia)
?I did an assay using RNA/DNA shield and it works perfect. The product have excellent reproducibility when stored at 4?C for up to a month.?
– A. Newhart (The Wistar Institute)
?The yield and the purity of the RNA is something out of this world, about at least 50% more RNA and purity not less than 1.95, we never got [that] before with other vendors such Invitrogen, Qiagen, etc.?
– Ehab S. (University of Iowa)
?The Direct-zol RNA MiniPrep showed the highested recovery [of miRNA] from both cell culture and frozen post-mortem human brain tissue when compared to miRNeasy, mirVana and RNeasy Plus.?
– O.E. (University of Southern California)
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