R2100 / R2101 / R2102 / R2103 / R2104 / R2105
Q1: Is Direct-zol suitable for very small numbers of cells?
Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)
Q2: Is DNase I available for individual purchase?
All kit components are available for purchase separately.
Q3: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q4: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q5: Is the kit compatible with samples stored in DNA/RNA Shield?
Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30?C). Add 3 volume of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.
Q6: Is it possible to extract proteins with the Direct-zol RNA kits?
Yes, proteins can be Acetone Precipitated post RNA binding step. Please request supplementary protocol from Zymo Research Technical Support.
Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?
Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?
Direct-zol DNA/RNA (D2080) kits can isolate DNA from TRIzol
Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?
Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.
Q10: Which phenol-based reagents are compatible with Direct-zol?
The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.
Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?
Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.
Q13: I ran out of RNA Wash Buffer. Can I use something else?
Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
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Epstein?Barr virus-encoded EBNA2 alters immune checkpoint PD-L1 expressi…
‘PD-L1 (E1L3N, cat# 13684) and p21 (#2947) and BCL2 (#15071) were purchased from Cell Signaling.. Total RNA from cell lines was isolated using Direct-zol RNA MiniPrep Plus kit (Zymo Research) according to the vending company?s instructions.. The integrity of RNA was routinely checked using 1% agarose gel and RNA quantification was estimated with a DS-11 spectrophotometer (DeNovix) [ ].’
Optimizing PCR Detection of Zika Virus from Various Body Fluids
‘?80?C freezing steps were tested: 1) right after spiking (then UCB added while still frozen and thawed with constant mixing); and 2) freezing the UCB pellet. . Additional procedures and reagents/supplies were tested to improve viral RNA recovery from semen and urine samples, including buffers and columns of RNeasy Plus Mini Kit or QIAamp Viral RNA Mini Kit, QIAzol, QIAshredder columns, ATL buffer (all QIAGEN), Direct-zol RNA MiniPrep Kit (Zymo Research), RNasin Plus RNase Inhibitor (Promega, Madison, WI), SUPERase In RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA), Protector RNase Inhibitor (Roche, Indianapolis, IN), ProtectRNA RNase Inhibitor (Sigma-Aldrich, St. Louis, MO), and Aptima Urine Specimen Transport Tubes (Hologic, Inc., Marlborough, MA).. Five microliters of extracted RNA were used in a duplicate 20-?L reaction with TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) on ViiA ‘
Diseases caused by different mutations in the two alleles of a gene are …
‘The FAH antibody used in IHC (Abcam, Cat. No. ab81087) was 1:400 diluted.. An aliquot of tissue powder was used to extract total RNA using the Direct-zol RNA Miniprep kit (Zymo Research, Cat. No. R2052).. RNA was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Fisher Scientific, Cat. No. 43-688-13), and subjected to PCR with ‘
?Previously I used a protocol that took 3 hours, now I can have my RNA in 20 minutes. What is not to like about that? Just one column and two buffers, I love it.?
– Arjan V. (Indiana University)
?Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years.?
– H.Z. (Harvard Medical School)
?This kit is amazing, I’ve got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!?
– R.K. CSU