Specifications
Description | Cells were derived from the recurrent CWR22 xenograft in nude mice. CWR-R1 cells have a functional androgen receptor (AR) that has a single mutation (AR-H874Y) that broadens the ligand specificity and is activated at low concentrations of testosterone or dihydrotestosterone (DHT). The CWR-R1 cell line is a co-culture of epithelial cells and stromal fibroblasts that grow as a monolayer. |
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SKU | T8263 |
Species | Human (H. sapiens) |
Tissue/Organ/Organ System | Prostate |
Donor Gender | Male |
Donor Disease | Prostate carcinoma |
Growth Properties | Adherent |
Cell Morphology Specification | Epithelial and fibroblast |
Seeding Density | 100,000 cells/cm2 |
Population Doubling Time | 24 hours |
Applications | For Research Use Only |
Unit quantity | 1×106 cells / 1.0 ml |
Cell Type | Tumor Cells |
Propagation Requirements |
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below. The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 5%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, and HEPES to a final concentration of 10 mM. |
Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase. |
QC | 1) STR profiling |
Disclaimer |
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Depositor | UNC |
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Shourideh, M., DePriest, A., Mohler, J. L., Wilson, E. M., & Koochekpour, S. (2016). Characterization of fibroblast-free CWR-R1ca castration-recurrent prostate cancer cell line. The Prostate, 76(12), 1067–1077. https://doi.org/10.1002/pros.23190