- High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
- Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
- Unique column design: Zero buffer retention and low (30 µl) elution volume.
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
|Applicable For||Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, transfections, and other sensitive applications.|
|Elution Volume||≥ 30 µl|
|Endotoxin Levels||< 50 EU/µg|
|Processing Time||15 min|
|Purity||Typical Abs260/280 ≥1.8.|
|Size Range||Up to 25 kb|
|Yield||Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.|