The cfKapture™ 21 kit is a magnetic bead-based DNA extraction kit designed to isolate circulating cell free DNA (cfDNA) from human plasma and serum. The cfKapture™ 21 kit is uniquely equipped with a plasma stabilization buffer; SE buffer allows plasma sample to be stabilized during storage or transport for up to 21 days at room temperature when mixed with plasma sample. This functionality offers processing laboratories time flexibility for optimizing their operational efficiency as well as outreach for receiving samples at local and international levels. The isolated cfDNA can be directly used for real time-PCR and DNA library preparation suitable for next generation sequencing.
Kit Components
- CFL Buffer
- SE Buffer
- CFW1 Buffer
- CFW2 Buffer
- Elution Buffer
- DF Solution
- Pro K Solution
- MAG-CFB Particles
Storage
Store MAG-CFB Particles, Pro K Solution, DF Solution at 2-8°C | DO NOT FREEZE. Store all other components 15-25°C.
Stability
Stable for 14 months when all components are stored correctly.
Disclaimers
For Research Use Only. Not for Use in Diagnostic Procedures.
Q1: Is the kit compatible to Streck tubes and PAXgene cfDNA tubes?
Yes, both Streck DNA and PAXgene tubes are compatible. First pellet down samples, remove the supernatant, and proceed with the protocol.
Q2: How to process samples stabilized in DNA/RNA Shield with the kit?
This kit is not compatible with samples stored in DNA/RNA Shield.
Reviews
There are no reviews yet.