Specifications
Description | CCOC-T cells were derived from clear cell odontogenic carcinoma tissue. The cells has a very slow growth rate however it is very invasive. The cell line expressed odontogenic factors, and epithelial mesenchymal transition (EMT)-related molecules. CCOC-T cells may be used for in vitro investigation of malignant odontogenic tumors. |
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SKU | T8241 |
Species | Human (H. sapiens) |
Tissue/Organ/Organ System | Oral |
Donor Gender | Female |
Donor Ethnicity | Asian |
Donor Age | 64 years old |
Donor Disease | Clear cell odontogenic carcinoma |
Growth Properties | Adherent |
Cell Morphology | Epithelial-like |
Population Doubling Time | 30 – 40 hours |
Applications | For Research Use Only |
Unit quantity | 1×106 cells / 1.0 ml |
Cell Type | Tumor Cells |
Propagation Requirements |
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below. The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. |
Subculture Protocol | 1. Wash with 1X PBS. 2. Add 1 ml of 0.05% Trypsin-EDTA. 3. Incubate for 2-3 minutes at room temperature. 4. Neutralize with 3 ml of complete growth medium. 5. Centrifuge cells at 120xg for 2 minutes. 6. Plate in ECM-coated dishes. If cell density is low, cells do not grow well. Therefore, please keep appropriate cell density for plating (1:2 or 1:3 split ratio). |
Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase. |
Disclaimer |
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Depositor | Tokushima University |
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