Our primer/probe sets are designed for specific detection of pathogens mentioned below and are separated into 4 multiplex qPCR setups. An additional primer/probe set to detect the human TERT gene (as an internal control) is also included. DNA extracted from respiratory specimens is amplified in a Real-Time PCR Thermocycler (such as Applied Biosystems 7500 Real-Time PCR Instrument). The fluorescent signal is emitted when the 5’ nuclease active domain of Taq polymerase degrades the probe anneals to the target sequence between the forward and the reverse primers. During each cycle, the fluorescence intensity is increased with the additional reporter dye molecules released from their respective probes.
The UTI) Pathogen Detection Assay also contains a positive control (Plasmid DNA) and a negative control for testing process monitoring.
SUMMARY AND EXPLANATION OF THE TEST
The UTI Pathogen Detection Assay is a real-time PCR test intended for the
qualitative detection of DNA from the bacteria and fungus in urine and blood
The assay includes the following urinary track bacteria targets:
Ureaplasma parvum serovar and Ureaplasma urealyticum
C. albicans; C. glabrata; C. parapsilosis and C. tropicalis
REAGENTS AND MATERIALS PROVIDED
|100 Tests / Box||500 Tests / Box|
|1||UTI PCR Mix||5 x 1.5 mL each/ Each tube||5 x 6.5 mL/ Each tube||14 µL|
|2||Enzyme Mix||550 µL/ tube x 1||550 µL/ tube x 1||1 µL|
|3||UTI Positive Control||50 μL/ tube × 1||250 μL/ tube × 1||5 μL|
|4||UTI Negative Control||50 μL/ tube × 1||250 μL/ tube × 1||5 μL|