The primer/probe sets are designed for the specific detection of pathogens mentioned below and are separated into 4 multiplex qPCR setups. An additional primer/probe set to detect the human TERT gene (as an internal control) is also included. DNA extracted from respiratory specimens is amplified in a Real-Time PCR Thermocycler (such
as Applied Biosystems 7500 Real-Time PCR Instrument). The fluorescent signal is emitted when the 5’ nuclease active domain of Taq polymerase degrades the probe anneals to the target sequence between the forward and the reverse primers. During each cycle, the fluorescence intensity is increased with the additional reporter dye molecules released from their respective probes.
The Pneumonia Panel Assay also contains a positive control (Plasmid DNA) and a negative control for testing process monitoring.
The Pneumonia Panel Assay is a real-time PCR test intended for the qualitative detection of DNA from the bacteria in nasopharyngeal or oropharyngeal swabs, sputum. The assay includes the following respiratory track bacteria targets:
Klebsiella pneumonia group
REAGENTS AND MATERIALS PROVIDED
|100 Tests / Box||500 Tests / Box|
|1|| Pn1 PCR Mix|
Pn2 PCR Mix
Pn3 PCR Mix
Pn4 PCR Mix
|1.5 mL each||7.5 mL each||14 µL|
|2||Enzyme Mix||450 µL/ tube x 1||450 µL/ tube x 1||1 µL|
|3||Positive Control||50 μL/ tube × 1||250 μL/ tube × 1||5 μL|
|4||Negative Control||50 μL/ tube × 1||250 μL/ tube × 1||5 μL|