The purpose of this protocol is to describe the procedure used for the detection of Monkeypox virus DNA in clinical specimens by real-time PCR. This assay detects DNA at varying concentrations, providing a qualitative
result of either positive, negative, or inconclusive in the identification of Monkeypox virus infections. Primer and probe are designed to detect Monkeypox genes. The assay kit also contains a positive control (Plasmid DNA) and a negative control for testing process monitoring.
Features:
- Rapid detection of pathogens in 2-3 hours.
- Higher accuracy when compared to traditional culture methods
- Higher sensitivity allows the detection of difficult-to-culture or slow-growing pathogens.
- Automated high-throughput formats enable testing labs to run thousands of samples per day.
- CE-IVD certified
- RUO in the USA
REAGENTS AND MATERIALS PROVIDED
| Item | Component | Quantity | Volume | |
| 100 Tests / Box | 500 Tests / Box | |||
| 1 | 1 MPXV PCR Mix | 1.5 mL each | 7.5 mL each | 14 µL |
| 2 | Enzyme Mix | 110 µL | 550 µL | 1 µL |
| 3 | Positive Control | 50 μL/ tube × 1 | 250 μL/ tube × 1 | 5 μL |
| 4 | Negative Control | 50 μL/ tube × 1 | 250 μL/ tube × 1 | 5 μL |
Reviews
There are no reviews yet.