Integrative analysis of DNA methylation, mRNAs, and small RNAs during ma?
Double-stranded DNA was denatured and immunoprecipitated using an anti-5-methycytosine monoclonal antibody (Zymo Research, Orange, CA, USA), and 3?dA-tailing using the Paired-end DNA Sample Prep Kit (Illumina, San Diego, CA, USA), the DNA samples were ligated to Illumina sequencing primer adaptors. . Double-stranded DNA was denatured and immunoprecipitated using an anti-5-methycytosine monoclonal antibody (Zymo Research, Orange, CA, USA).. 220 bp to 320 bp bands were excised and purified from the immunoprecipitation gel and quantified using an Agilent 2100 Analyzer (Agilent Technologies
Identification of MicroRNA-124-3p as a Putative Epigenetic Signature of ?
Two micrograms of total gDNA isolated from rat PFC was treated with RNaseA and sheared to generate a random fragment length of ~400 bp.. Fragmented gDNA was first denatured and then was conjugated using Protein G magnetic beads (EMD Millipore) and 5 ?g monoclonal 5-methyl cytosine antibody (Zymo Research, Irvine, CA).. A portion of (10%) untreated sonicated denatured gDNA was kept separately as input control.’
- Catalog#: A3002-15 /A3002-200 /A3002-30 A3002-50
- Package Length (in Inches): 1.8
- Package Width (in Inches): 0.4
- Package Height (in Inches): 0.4
- Package Weight (in Pounds): 0.1
- Size: 15 ?l /200 ?l /30 ?l /50??l
- Unit Standard: Metric
- Volume Units: Milliliters