All-In-One 5X RT MasterMix

From RNA template to cDNA, completely hassle-free

All-In-One 5X RT MasterMix is a convenient and ready-to-use formulation for first-strand cDNA synthesis, including genomic DNA (gDNA) removal. Genomic DNA contamination is a common problem for accurate RNA detection and this MasterMix solves that problem without affecting reverse transcription and first-strand cDNA synthesis. cDNA synthesis will be simple, reliable, and reproducible. The optimized 5X RT MasterMix contains abm’s proprietary OneScript^® Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a finely-balanced ratio of Oligo (dT)s and Random Primers. The high-quality cDNA synthesized from this kit can be used for a wide range of downstream applications.

Product Features:

• Easy one-step setup reduces pipetting and sample handling
• High cDNA yields from difficult samples with OneScript^® Hot Reverse Transcriptase (Cat. No. G593)
• Inhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (Cat. No. G591)
• Removes contaminating gDNA with temperature-sensitive DNase
• Also includes dNTPs, Oligo (dT)s, and random primers
• Earn 2X the rewards points

• Generation of templates for use in RT-PCR and qRT-PCR
• cDNA synthesis from ssRNA
• cDNA library construction
• Generation of probes for hybridization
• DNA primer extension

Store at -20°C.