|Description||PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 14 bands, ranging from 250 pb – 25 kb, for molecular weight standards in agarose gel electrophoresis. The 1,000 and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size. Total DNA concentration for all bands combined is 103.2ng/μl.|
|Applications||For approximating DNA size and amount on agarose gels.|
|Note||Concentration of this marker is 104 μg / ml|
|Suggested Load||Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel.|
|Shipping Conditions||Shipped on blue ice packs.|
|Storage Condition||Store @ 4°C for up to 3 months or -20°C for up to 24 months.|
|Unit quantity||500 μl/100 loads|
Q.1: Is it ready for use?
Yes, it is ready to use. Just load suggested volume (5ul) to the well.
Q.2: What is the smallest size agarose gel can separate?
That is around 300bp for 1% agarose and 100bp for 2.5% agarose. With anything smaller than the dye, there may be separation but it will be difficult to tell when the fragment will run off the gel.
Q.3: Are the Opti-DNA markers compatible with radiolabelling?
Our lab has not tested the Opti-DNA markers for this particular application, but we believe it shouldn’t be a problem. The Opti-DNA markers should be compatible for radio-labelling.