MCDB media were developed for the culture of specific cell types without a serum supplement. MCDB 153 medium is a modification of Ham’s nutrient mixture F-12 designed specifically for the growth of human epidermal keratinocyte culture, clonal growth of chicken embryo fibroblasts, or Chinese Hamster Ovary (CHO) cells.
MCDB 153 medium includes trace elements, L-glutamine, sodium pyruvate, and HEPES for more effective buffering capacity and energy sources. The details are listed in the formulation. The main modifications are below:
- Sodium Pyruvate
- Phenol Red
- Sodium Bicarbonate
- Insulin, transferrin, and sodium selenite
- Human epidermal keratinocyte
- Chinese Hamster Ovary (CHO)