PCR Master Mix
PCR Master Mix is a Thermo scientific 2X concentrated solution. It comprises dNTPs, Taq DNA Polymerase, and all the elements that are required to conduct PCR except primers and DNA templates. The already mixed amalgamation helps to save time and also reduce contamination, which is due to a decreased number of pipetting steps required in a PCR setup. The Master Mix is optimized to exhibit efficiency and reproducible PCR. It is a convenient, ready-to-use mix. It is thermo-stable, which means that its half-life is more than 40 minutes at a temperature of 95Ā°C. It incorporates modified nucleotides, such as digoxigenin, biotin, and fluorescent-labeled nucleotides. The error rate in PCR of Taq DNA Polymerase is 2.2 x 10-5 errors per NT per cycle. The accuracy rate of PCR is 4.5 x 104. The accuracy rate is an inverse of the error rate, which shows the average number of correct nucleotides infused prior to the occurrence of an error.
