Get the probe-based qPCR master mix for your laboratory from MBP Inc.’s collection and get accurate results on your samplings!
Probe-based qPCR master mixes are universal, ready-to-use solutions that are optimized for qPCR. These master mixes come with the Taq DNA polymerase, which makes sure that the PCR specificity and sensitivity. dUTP and uracil DNA glycosylase (UDG) are included in the mixture for carry-over contamination control. These master mixes are used for straightforward quantitative PCR or real-time PCR reactions. There’s a given mix that has polymerase, buffer, dNTPs, MgCl2, and fluorescent dye.
In concentrations from 2X to 5X, it is ready to use with the addition of a template and primers. For RNA templates, RT-qPCR kits that have reverse transcriptase are available in ‘one-step’ or ‘two-step’ formats.
Uses Of Probe-Based qPCR Master Mix:
Probe-based qPCR master mixes are used for many reasons:
Probe-based quantitative PCR (qPCR) uses real-time fluorescence from 5’-3’ exonuclease cleavage of a fluorescently label with the target-specific probe to measure DNA amplification at each cycle of a PCR.
Probe-based qPCR is typically more specific than dye-based qPCR. The foundational technology is employed in qPCR diagnostic assays.
Probe designs vary, but the most common type of hydrolysis probes have incorporated a 5’ reporter fluorophore and a 3’ quencher on a short oligonucleotide complementary to the target sequence.
Fluorescence resonance energy transfer (FRET) prohibits the emission of the fluorophore while the oligo probe is intact.
Probe-based qPCR allows multiple targets to be quantified in a single reaction (multiplexing) by using a unique fluorescent dye for each amplicon-specific probe.
Probe-based qPCR master mixes are highly compatible with the following:
- Sequence Detection System
- Real-Time PCR System
- A Thermal Cycler
- SmartCycler System
- Real-Time Rotary Analyzer
- Multiplex Quantitative PCR System
What are you waiting for now? Add these probe-based qPCR master mix kits to your laboratory. Check it out at MBP Inc.’s collection today!