Get the best-in-class and high-quality Taq DNA Polymerase for your laboratory from MBP Inc.’s collection!
Taq DNA polymerase is the most common enzyme used for PCR amplification. This enzyme is extremely heat-resistant and has a half-life of 40 minutes at 95 degrees Celsius. At the optimal temperature, which is 72 degrees Celsius, the nucleotides are incorporated at a rate of 2–4 kilobases per minute.
Polymerases are active over a broad temperature range (Gelfand and White, 1990). If DNA, primers, and Taq polymerase are mixed, then the mispriming and elongation can occur at room temperature.
You can order Taq Polymerase (PCR Enzymes) for your use or your laboratory’s use from MBP Inc. to get accurate results!
Uses of Taq Polymerase:
DNA Amplification: Taq polymerase is a crucial component in PCR, where it synthesizes new DNA strands by extending primers along a DNA template. This enzyme can withstand the high temperatures (around 70-75°C) needed for DNA denaturation, making it suitable for PCR.
DNA Cloning: Taq polymerase is used in the amplification of specific DNA fragments for subsequent cloning into plasmids or other vectors, a common technique in genetic engineering.
Advantages of Taq Polymerase:
Thermal Stability: Taq polymerase is highly heat-stable and can withstand the high-temperature cycles used in PCR. This property is due to its origin in a thermophilic bacterium. It can endure the denaturation step (usually around 95°C) without becoming denatured itself.
Single-Step Reaction: Unlike some other DNA polymerases, Taq polymerase has 5′ to 3′ exonuclease activity, which means it doesn’t have proofreading capabilities. While this can lead to a higher error rate in DNA replication, it simplifies PCR reactions and makes them suitable for certain applications, such as mutation detection.
Convenience: Taq polymerase is widely available commercially and is relatively affordable, making it accessible for many researchers.
Versatility: Taq polymerase is used in a variety of PCR applications, including standard PCR, colony PCR, RT-PCR (reverse transcription PCR), and more.
What are you waiting for now? Place an order for your Taq DNA Polymerase from MBP Inc.’s collection for your laboratory today!