RNase Inhibitor, also known as Ribonuclease Inhibitor, is a protein that is used in molecular biology and biochemistry to protect RNA molecules from degradation by ribonucleases (RNases).
RNases are enzymes that break down RNA into smaller fragments, and they can be present in laboratory environments or biological samples. RNase Inhibitor is derived from human placenta or recombinant sources.
Many commercially available RNase inhibitors are designed for protecting sensitive, RNA-based experiments. Placental ribonuclease inhibitor is a 50kDA protein that inhibits RNase by binding it in a 1:1 ratio with an association constant that is greater than 1016 M–1
It is expressed as a single-chain polypeptide that has 460 amino acids, with protein: protein interactions. RNasin Ribonuclease Inhibitor is a non-competitive inhibitor of RNases A, B, and C, human placental RNase, and angiogenin. Most of the RNasin ® Ribonuclease inhibitors affect a broad spectrum of RNases. You can add RNase Inhibitors to your laboratory and get them from MBP Inc.
Uses of RNase Inhibitor:
Protecting RNA samples: When working with RNA in the laboratory, it’s crucial to prevent RNA degradation. Adding RNase Inhibitors to RNA samples or reactions helps safeguard the integrity of the RNA molecules.
In vitro transcription: In experiments involving in vitro transcription, where RNA is synthesized from a DNA template using RNA polymerases, an RNase Inhibitor is often added to protect the newly synthesized RNA.
Reverse transcription: In reverse transcription reactions, where RNA is converted into complementary DNA (cDNA), RNase inhibitors can be included to preserve the RNA template.
PCR and RT-PCR: RNase inhibitors can be used in polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR) to prevent RNA degradation during these processes, especially when dealing with RNA templates.
RNA purification and storage: When purifying RNA from samples or storing RNA for future use, RNase Inhibitor is added to maintain RNA integrity and prevent degradation during handling and storage.
Order your RNase inhibitors for your work purposes and sampling from MBP Inc.’s collection today!
