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You can get the best DNA ligase for your laboratory from MBP Inc.’s collection.

MBP Inc. renowned as the best supplier in Canada & USA provides Ligase. Ligase is any class of about 50 enzymes that catalyze reactions involving the conservation of chemical energy. It will provide a couple between energy-demanding synthetic processes and energy-yielding breakdown reactions.

They catalyze the joining of two molecules, deriving the needed energy from the cleavage of an energy-rich phosphate bond. You can get ligases for modifying enzymes. Get them from MBP Inc.’s collection.

Types of DNA Ligase

The DNA ligase enzyme has many forms in different organisms, which include:

  • coli DNA ligase: This will utilize nicotinamide adenine dinucleotide (NAD) to form a phosphodiester bond between DNA strands. It doesn’t join strands with blunt ends. Furthermore, the performance of E. coli DNA ligase can be enhanced under in vitro conditions by adding DNA polymerase in the right amount to the reaction mixture.
  • T4 DNA ligase: This is isolated from bacteriophages and is one of the most commonly used DNA ligases for research purposes. It can join oligonucleotides, blunt and cohesive ends of DNA, RNA, and RNA/DNA hybrids.

Unlike E. coli, T4 DNA ligase uses adenosine triphosphate (ATP) as a cofactor rather than NAD.

  • Mammalian DNA ligase
  • Ligase I: After ribonuclease H removes the RNA primer from the Okazaki fragments, DNA ligase I joins the nascent DNA.‍
  • Ligase II: It is produced after DNA ligase III is degraded by proteases (an enzyme that breaks down long polypeptides into short amino acid fragments). ‍
  • Ligase III: The only ligase that is present in the mitochondria. It facilitates DNA repair by nucleotide excision repair in association with XRCC1 protein.‍
  • Ligase IV: It is connected with the XRCC4 protein and facilitates the repair of double-stranded DNA breaks through the non-homologous end-joining (NHEJ) pathway.‍
  • Thermostable DNA ligase: It is found in a thermophilic bacterium and can withstand a higher temperature of 95 Celsius, unlike all other DNA ligases.