At MBP Inc, we offer a wide variety of unique and affordable assortment of specialty enzymes. These enzymes can be used for epigenetic, genetic, and expression-based applications.
For many experimental procedures such as transformation, protein purification, spheroplasting, and immunofluorescence, the digestion of yeast and fungal cell walls is extremely important.
For digestion, the usage of lytic enzymes such as Zymolyase is usually implied. Zymolase is prepared from Arthrobacter luteus, lyophilized, and it is packaged along with a resuspension buffer. The buffer has undergone optimization so that it can confer maximized levels of enzymatic activities. The most prominent activities of enzymes are β-1,3 glucanase and β-1,3-glucan laminaripentao-hydrolase. They hydrolyze the glucose polymers at β-1,3-glucan linkages, which release laminaripentao as their fundamental products. The optimal Zymolyase activity takes place at 30° – 37°C. The lytic activity halts at temperatures higher than that. R-Zymolyase also includes 0.5U/ul RNase A at the time of its reconstitution.